In the Jones lab, we use protein film electrochemistry to probe the inner workings of redox proteins and enzymes. Protein film electrochemistry has proven to be a versatile and efficient method which can be tailored to address both thermodynamic and kinetic questions. We have used this technique to study a myriad of different enzymes ranging from [NiFe]-hydrogenases to a multi-subunit bifurcating enzyme known as Nfn. Furthermore, we are developing methods to quantify and simulate the catalytic profiles of these enzymes so that their kinetic and thermodynamic properties can be quantified.
Artz, J. H.; Mulder, D. W.; Ratzloff, M. W.; Lubner, C. E.; Zadvornyy, O. A.; LeVan, A. X.; Williams, S. G.; Adams, M. W. W.; Jones, A. K.; King, P. W.; Peters, J. W., Reduction Potentials of FeFe -Hydrogenase Accessory Iron-Sulfur Clusters Provide Insights into the Energetics of Proton Reduction Catalysis. Journal of the American Chemical Society 2017, 139 (28), 9544-9550.
Cereda, A.; Hitchcock, A.; Symes, M. D.; Cronin, L.; Bibby, T. S.; Jones, A. K. A Bioelectrochemical approach to characterize extracellular electron transfer by synechocystis sp. PCC6803. PLOS One 2014, 9, e91484.
Ashur, I.; Schulz,O.; McIntosh, C.; Pinkas, I.; Ros, R.; Jones, A. K. Transparent gold as a platform for unmediated protein spectroelectrochemistry: investigation of cytochrome c and azurin. Langmuir 2012, 28, 5861-5871.
Ashur, I.; Jones, A. K. Immobilization of azurin with retention of its native electrochemical properties at alkylsilane self-assembled monolater modified indium tin oxide.. Electrochimica Acta 2012, 85, 169-174.
Kwan, P.; Schmitt, D.; Volosin, A. M.; McIntosh, C. L.; Seo, D. K.; Jones, A. K. Spectroelectrochemistry of cytochrome c and azurin immobilized in nanoporous antimony tin oxide. Chemical Communications 2011, 47, 12367-12369.
McIntosh, C.L.; Germer, F.; Schulz, R.; Appel, J.; Jones, A. K. The [NiFe]-hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 works bidirectionally with a bias to H2 production. Journal of the American Chemical Society 2011, 133, 11308-11319.